HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V

HPLC Analysis of Polyglutamate Metabolites of Methotrexate on TSKgel® NH2-100 and ODS-100V

Conditions

column TSKgel NH2-100, 15 cm x 2 mm I.D., 3 µm particles (821968)
column TSKgel ODS-100V, 15 cm x 2 mm I.D., 3 µm particles (821810)
column temp. 40 C
mobile phase for TSKgel ODS-100V: [A] water:acetonitrile (90:10) + 0.1% TFA; [B] water + 0.1% TFA
mobile phase for TSKgel NH2-100: [A] water:acetonitrile (10:90) + 0.1% TFA; [B] water + 0.1% TFA
gradient 0 to 40% B in 15 min; to 0% B in 2 min
flow rate 0.2 ml/min
injection 10 µl
detector UV, 313 nm

Description

Analysis Note Polyglutamate metabolites of methotrexate (MTXPG2~7) were separated on TSKgel NH2-100 and TSKgel ODS-100V narrow bore columns. MTX polyglutamate metabolites were eluted in the order of the number of glutamate groups in their molecules on the TSKgel NH2-100 column. Despite the early elution of MTXPG1 and MTXPG2 on the TSKgel NH2-100 column, the overall separation is superior over what can be accomplished on the C18 column.
Categories Analytical Chromatography, Antineoplastics
Featured Industry Clinical
Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
Related Links