HPLC Analysis of Procyanidins on Ascentis® Phenyl

HPLC Analysis of Procyanidins on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate, (pH 3.0 with concentrated formic acid): [B] acetonitrile; (85:15, A:B)
flow rate 1.0 mL/min split to the MS
sample 10 mg/mL in 85:15, 10 mM ammonium formate, (pH 3.0 with concentrated formic acid): acetonitrile
injection 10 µL
detector MS, ESI (-) in Single Ion Recording (SIR) Mode

Description

Categories Analytical Chromatography, Flavors, Fragrances
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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