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HPLC Analysis of Proline Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

HPLC Analysis of Proline Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

Conditions

column CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST)
column temp. 25 C
mobile phase [A] water; [B] methanol; [C] formic acid; (40:60:0.02, A:B:C)
flow rate 1 ml/min
sample 200 mug /mL in 50:50 water:methanol
injection 10 µl
detector UV, 205 nm

Description

Categories Analytical Chromatography, Amino Acids
Featured Industry Life Science and Biopharma
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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