HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

Conditions

column BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 µm particles (66825-U)
mobile phase (A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile]
gradient 25 to 65% B in 8 min
flow rate 0.3 ml/min
pressure 1430 psi (99 bar)
sample protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4
injection 1 µl
detector UV, 215 nm

Description

Analysis Note This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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