Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

Conditions

column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column temp. 25 C
mobile phase [A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)
flow rate 0.3 ml/min
sample in mobile phase
injection 2.5 µl
detector 1000 psi
detector UV, 280 nm

Description

Analysis Note This application compares selectivity of proteins when chromotographed by size exclusion with different buffer systems.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
Related Links