HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

Conditions

column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm (Z777033)
column temp. 25 °C
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid
flow rate 0.1 mL/min
pressure 173 psi (12 bar)
sample protein standards
injection 4 µL
detector UV, 215 nm

Description

Analysis Note Due to the low diffusion coefficents of proteins, optimal flow in size exclusion chromatography is often very low. However, the disadvantage is long run times. This example demonstrates both features.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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