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HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine

Conditions

column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (A777028)
column temp. 25 C
mobile phase [A], 0.15 M potassium phosphate, monobasic, pH 7.0 with potassium hydroxide; [B] 0.15 M potassium phosphate, monobasic, 0.2 M arginine, pH 7.0 with phosphoric acid (mobile phase is either 100% A or 100% B)
flow rate 0.3 ml/min
pressure 1030 psi100% A (71 bar)
pressure 1165 psi100% B (80 bar)
sample Waters BEH200 SEC protein standard
injection 2.5 µl
detector UV, 280 nm

Description

Analysis Note Arginine can have benefitial effects on peak shape and recovery of hydrophobic proteins when chromatographed by size exclusion chromatography. In this case, with protein standards that are not particularly hydrophobic, no dramatic affect is noted, as assessed by peak efficiency, peak widths, or peak retention.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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