HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Potassium Chloride

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Potassium Chloride

Conditions

column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column temp. 25 °C
mobile phase [A], 0.15 M potassium phosphate, monobasic, pH 7.0 with potassium hydroxide; [B] 0.15 M potassium phosphate, monobasic, 0.2 M potassium chloride, pH 7.0 with potassium hydroxide; (mobile phase is either 100% A or 100% B)
flow rate 0.3 mL/min
pressure 1000 psi (69 bar)
sample Waters BEH200 SEC protein standard
injection 2.5 µL
detector UV, 215 nm

Description

Analysis Note In size exclusion chromatography, addition of halide salts (typically sodium or potassium chloride) in modest concentration, to an aqueous buffer, can yield improved chromatographic performance with protein analytes. In this case, as regards chromatographic performanc, little impact is noted except in the case of IgG, where peak shape is improved.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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