Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Protein Tryptic Digest on Ascentis® RP-Amide

HPLC Analysis of Protein Tryptic Digest on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 25 cm x 2.1 mm I.D., 5 µm particles (565306-U)
column temp. ambient
mobile phase [A] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:water; [B] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:acetonitrile
gradient 10 to 50% B in 80 min
flow rate 0.2 ml/min
sample tryptic digest of carboxymethylated protein mixture 1.5 g/L in 50 mM formic acid
injection 4 µl
detector ESI (+)

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links