HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

Conditions

column BIOshell A400 Protein C4, 10 cm X 2.1 mm I.D., 3.4 µm particles (66825-U)
column temp. 30 C
mobile phase [A] 75:25, (0.1% TFA in water):(0.1% TFA in acetonitrile) ; [B] 25:75, (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient 15 to 75% B in 10 min
flow rate 0.3 ml/min
pressure 2410 psi (166 bar)
sample protein mixture, g/L ea: superoxide dismutase, 0.3; bovine serum albumin, 0.3; alpha-lactalbumin, 0.2; beta-lactoglobulin A, 0.3; catalase, 0.3; (carbonic anhydrase, 0.3; beta-Amylase, 1.0; glucose-6-phosphate dehydrogenase, 1.0; thioredoxin, 0.3; phosphorylase b, 2.5.)
injection 1 µl
detector UV, 215 nm

Description

Analysis Note This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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