HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

Conditions

column Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 µm particles (59602-U)
mobile phase [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate 0.5 mL/min
injection 10 mug trypsin inhibitor, 5 mug conalbumin, myoglobin or 10 mug ribonuclease A, 5 mug cytochrome c, a-chymotrypsinogen A
detector UV, 280 nm

Description

Analysis Note The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
description Separated on Discovery® BIO PolyMA-WAX
suitability application for HPLC

Materials

     
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