HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

Conditions

column Proteomix WCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 µm particles (Z777180)
column Proteomix WCX NP3, 5 cm x 4.6 mm I.D., 3 µm particles (Z777185)
column Proteomix WCX-NP10, 5 cm x 4.6 mm, 10 µm particles (Z777169)
column Proteomix WCX-NP5, 5 cm x 4.6 mm x 5 µm particles (Z777193)
column temp. ambient
mobile phase (A) 20 mM sodium phosphate, pH 6.5, (B) A + 1.0 M NaCl
gradient 0 to 100% B in 25 min, 15 min prewash
flow rate 0.5 mL/min
sample Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL) min
injection 5 µL
detector UV, 214 nm

Description

Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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