Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

Conditions

column TSKgel Chelate-5PW, 5 cm x 5 mm I.D., 10 µm particles (column saturated with Zn2+ ions) (814440)
mobile phase [A] 20 mM HEPES, 0.5 M sodium chloride, 1 mM imidazole, pH 8 with sodium hydroxide; [B]  20 mM HEPES, 0.5 M sodium chloride, 20 mM imidazole, pH 8 with sodium hydroxide
gradient 30 min linear gradient from 1 mM to 20 mM imidazole in 20 mM HEPES-NaOH buffer, pH 8 containing 0.5 M NaCl
flow rate 0.8 ml/min
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
Related Links