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HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size

HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size

Conditions

column Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 µm, 150 Angstrom particles (Z777018)
column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm, 300 Angstrom particles (Z777033)
column temp. ambient
mobile phase 150 mM sodium phosphate, pH 7
flow rate 1 ml/min
sample each 1 mg/mL except uracil, 2.5 mg/mL
injection 10 µl
detector UV, 214 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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