HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

Conditions

column Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm, 300 Angstrom particles (Z777028)
column temp. ambient
mobile phase 150 mM potassium phosphate, pH 6.8
flow rate as indicated on figure
sample each 1 mg/mL except uracil, 2.5 mg/mL
injection 10 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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