HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

Conditions

column BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (66905-U)
column temp. 35 °C
mobile phase [A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile
gradient held at 0% B for 0.5 min; 0 to 100% B in 7 min
flow rate 0.3 mL/min
pressure 4743 psi (327 bar)
sample 0.2 - 0.6 g/L in mobile phase A
injection 1 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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