HPLC Analysis of Proteins on SRT® SEC-2000, 30 cm x 7.8 mm I.D.

HPLC Analysis of Proteins on SRT® SEC-2000, 30 cm x 7.8 mm I.D.

Conditions

column SRT® SEC-2000, 30 cm x 7.8 mm I.D., 5 µm particles (Z777069)
column temp. 25 °C
mobile phase 150 mM potassium phosphate monobasic, pH 7.0
flow rate 1.0 mL/min
sample 1- 10 g/L in buffer
injection 10 µL
detector UV, 280 nm

Description

Analysis Note This application demonstrates the suitability of SRT SEC-2000 for the separation of proteins. Separation is by inclusion into the pore volume with larger molecules being excluded and therefore eluting earlier.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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