HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 4.6 mm I.D., 3 μm

HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 4.6 mm I.D., 3 μm

Conditions

column Zenix(R) SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777033)
column temp. 25 °C
mobile phase 150 mM potassium phosphate monobasic, pH 7.0 (adjusted with potassium hydroxide)
flow rate 0.25 mL/min
pressure 652 psi (45 bar)
sample 1 g/L each (except uracil, 0.1 g/L) in mobile phase
injection 1 µL
detector UV, 214 nm

Description

Analysis Note This application demonstrates the suitability of Zenix SEC-300 for the separation of proteins. Separation is by inclusion into the pore volume with larger molecules being excluded and therefore eluting earlier.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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