HPLC Analysis of Proteins on Proteomix® SCX-NP5

HPLC Analysis of Proteins on Proteomix® SCX-NP5

Conditions

column Proteomix SCX-NP5, 5 cm x 4.6 mm I.D., 5 µm particles (Z777159)
column temp. 25 °C
mobile phase [A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient 0 to 60% in 20 min
flow rate 0.3 mL/min
pressure 400 psitotal pressure (28 bar)
sample ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
injection 5 µL
detector UV, 250 nm

Description

Analysis Note The separation of four readily investigated proteins was examined on a Proteomix SCX-NP5 Column in order to examine retention time and resolution. The four peaks were well resolved in under 15 minutes.
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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