HPLC Analysis of Purines and Pyrimidines of Nucleic Acids on Ascentis® Si (Silica)

HPLC Analysis of Purines and Pyrimidines of Nucleic Acids on Ascentis® Si (Silica)

Conditions

column Ascentis Si, 10 cm x 2.1 mm I.D., 5 µm particles (581508-U)
column temp. 35 °C
mobile phase [A] 200 mM ammonium formate (pH 3.0, with formic acid): [B] acetonitrile; (10:90, A:B)
flow rate 0.2 mL/min
sample 100 µg/mL in mobile phase
injection 1 µL
detector UV, 270 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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