HPLC Analysis of Pyroglutamic Acid and an Impurity on SeQuant ZIC-HILIC

HPLC Analysis of Pyroglutamic Acid and an Impurity on SeQuant ZIC-HILIC

Conditions

column SeQuant ZIC-HILIC, 250 x 4.6 mm, 5 µm, 200 Ã… (1.50458.0001)
mobile phase [A] 10mM potassium phosphate pH 3; [B] acetonitrile
gradient 82% B held for 10 min; to 60% B in 10 min; held at 60% B for 5 min
flow rate 1.0 mL/min
pressure 696 psi (48 bar)
sample 1 mg/mLin mobile phase
injection 5 µL
detector 210 nm

Description

Featured Industry Chemicals and Industrial Polymers
Life Science and Biopharma
suitability application for HPLC

Materials

     
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