HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution

HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution

Conditions

column SRT SEC-300, 30 cm x 7.8 mm I.D., 5 µm, 300 Angstrom particles (Z777051) (Z777033)
column Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 µm, 300 Angstrom particles (Z777051)
mobile phase 150 mM sodium phosphate, pH 7.0
flow rate 1 ml/min
sample Recombinant tumor necrosis factor receptor-Fc fusion protein (rhTNFR-Fc), including oligomers (440 kD and 360 kD), monomer (150 kD), and impurity (120 kD)
injection 20 µl
detector UV, 214 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information SRT is a registered trademark of Sepax Technologies, Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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