HPLC Analysis of Separation of Human IgG Monomer, Half-body, and Fragments using a TSKgel® SuperSW mAb HR column

HPLC Analysis of Separation of Human IgG Monomer, Half-body, and Fragments using a TSKgel® SuperSW mAb HR column

Conditions

column TSKgel SuperSW mAb HR, 30 cm x 7.8 mm ID, 4 µm particles (822854)
column temp. 25 C
mobile phase 0.1 mol/L phosphate/0.1 mol/L sulfate buffer + 0.05% sodium azide
flow rate 0.5 ml/min
sample human IgG (4.6 g/L)
injection 10 µl

Description

Analysis Note Recent research has shown an interest in mAb half-bodies as therapeutic vectors as they can be further targeted for conjugation, enzyme labeling, or antibody immobilization. The superior resolution obtained between a monoclonal antibody monomer and half-body species using a TSKgel® SuperSW mAb HR column is demonstrated in this application.
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information TSKgel is a registered trademark of Tosoh Corporation
Other Notes Human IgG (4.6 g/L) was reduced by incubation with 150 mM tris(2-carboxyethyl)phosphine (TCEP) for 20 hours at 37 °C.
suitability application for HPLC

Materials

     
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