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HPLC Analysis of Sigma Protein Standard Mix on Zenix® SEC-300

HPLC Analysis of Sigma Protein Standard Mix on Zenix® SEC-300

Conditions

column Sepax Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 µm particles (Z777028)
column temp. 25 C
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate 0.3 ml/min
pressure 1050 psi (72 bar)
sample 0.5 mL of mobile phase added to one vial of  Sigma Protein Standard Mix   (p/n 69385); filtered 0.45 µm Millex-HV syringe filter (p/n Z227552)
injection 2.5 µl
detector UV, 215 & 280 nm

Description

Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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