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HPLC Analysis of Sigma Protein Standard Mix on SRT® SEC-500

HPLC Analysis of Sigma Protein Standard Mix on SRT® SEC-500


column Sepax SRT SEC-500, 30 cm x 4.6 mm I.D., 5 µm particles (Z777055)
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate 0.3 ml/min
pressure 580 psi (40 bar)
temp. 25 C
sample 0.5 mL of mobile phase added to one vial of  Sigma Protein Standard Mix (p/n 69385); filtered 0.45 µm Millex-HV syringe filter (p/n Z227552)
injection 2.5 µl
detector UV, 215 & 280 nm


Analysis Note Protein standards spanning a wide range of molecular weights can reveal the size range best resolved by a given gel filtration column. The Sepax 500Ã… SEC column provides good resolution of sample components ranging in molecular weight of ~150,000 to >1000,000.
Featured Industry Life Science and Biopharma
Legal Information SRT is a registered trademark of Sepax Technologies
suitability application for HPLC


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