HPLC Analysis of Silymarin on Ascentis® Express C18

HPLC Analysis of Silymarin on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U)
column temp. 35 C
mobile phase [A] water with 0.1% formic acid; [B] methanol
gradient held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate 0.6 ml/min
pressure 4105 psi (283 bar)
sample 100 µg/mL in 90:10, water:methanol
injection 20 µl
detector UV, 254 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of silymarin.
Categories Analytical Chromatography, Phenols
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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