HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 55:45)

HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 55:45)

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 °C
mobile phase [A] water; [B] acetonitrile; (55:45, A:B)
flow rate 1 mL/min
sample as indicated in 55:45, water:acetonitrile
injection 10 µL
detector UV, 220 nm

Description

Categories Analytical Chromatography, Clinical, Steroids, steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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