HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 60:40)

HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 60:40)

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (60:40, A:B)
flow rate 1 ml/min
sample 50 µg/mL each in mobile phase
injection 10 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Clinical, steroids, Steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links