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HPLC Analysis of Steroids on Ascentis® Express C18

HPLC Analysis of Steroids on Ascentis® Express C18


column Ascentis Express C18 10cm x 2.1mm, 2.7 µm particles (53823-U)
column temp. 40 C
mobile phase [A] water [B] acetonitrile (70:30, A:B)
flow rate 0.7 ml/min
pressure 5438 psi (375 bar)
sample 0.125 mg/mL each in 37.5/62.5 water/organic solvent
injection 2 µl
detector UV, 254 nm


Categories Analytical Chromatography, Steroids
Featured Industry Clinical
Pharmaceutical (small molecule)
General description This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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