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HPLC Analysis of Steroids on Ascentis® Express Biphenyl

HPLC Analysis of Steroids on Ascentis® Express Biphenyl


column Ascentis Express Biphenyl,  5 cm x 4.6 mm I.D., 2.7 µm particles (64059-U)
column temp. 30 C
mobile phase [A] water; [B] acetonitrile
gradient 20 to 60% B in 6 min
flow rate 1.85 ml/min
pressure 4998 psi (344 bar)
sample 25 ng/mL each except 125 ng/mL deoxycorticosterone in 62.5:37.5, water:acetonitrile
injection 4 µl
detector UV, 215 nm


Categories Analytical Chromatography, Steroids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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