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HPLC Analysis of Substance P and Its Fragments on Discovery® C18

HPLC Analysis of Substance P and Its Fragments on Discovery® C18

Conditions

column Discovery C18, 15 cm x 4.6 mm I.D., 5 µm particles (504955)
column temp. 35 C
mobile phase [A] water + 0.1% TFA; [B] acetonitrile + 0.1% TFA
gradient linear; 5-29%B (6 min); 29-34B (5 min)
flow rate 1 ml/min
sample Substance P in initial mobile phase
detector UV, 220 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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