HPLC Analysis of Substituted Aromatic Compounds on Ascentis® Express Biphenyl

HPLC Analysis of Substituted Aromatic Compounds on Ascentis® Express Biphenyl

Conditions

column Ascentis Express Biphenyl, 10 cm x 2.1 mm, 2.7 µm particles (64065-U)
column temp. 35 °C
mobile phase [A] Water; [B]Methanol; (57:43 A:B)
flow rate 0.5 mL/min
pressure 7900 psi (545 bar)
sample Uracil (25 µg/mL), Benzonitrile (50 µg/mL), Acetophenone (25 µg/mL), Anisole (100 µg/mL),
injection 1 µL
detector UV, 254 nm

Description

Analysis Note This application demonstrate the separation of substituted aromatic compounds on Ascentis Express Biphenyl. These analytes highlight the unique selectivity of this stationary phase for this type of analytes using methanol reversed phase conditions.
Categories Analytical Chromatography, Aromatics
Featured Industry Chemicals and Industrial Polymers
General Analytical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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