HPLC Analysis of Sugar Phosphates on SeQuant®ZIC-HILIC

HPLC Analysis of Sugar Phosphates on SeQuant®ZIC-HILIC

Conditions

column SeQuant ZIC-HILIC, PEEK, 150 x 4.6 mm, 5 μm, 200 Å (1.50455.0001)
mobile phase phase: [A] 40 mM potassium phosphate, pH 3.0; [B] acetonitrile; (35:65, A,B)
flow rate 1.0 mL/min
column temp. 40 °C
detector 210 nm
injection: 1 μL
sample D-ribulose 1,5-bisphosphate sodium salt, 0.07% (w/v); 2,3-diphospho-D-glyceric
acid penta sodium salt, 0.15% (w/v) in acetonitrile/water (60:40)

Description

Featured Industry Food and Beverages
Legal Information SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
Related Links