HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection

HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection

Conditions

column Ascentis Express OH-5, 15 cm x 4.6 mm, I.D., 2.7 µm particles (53778-U)
column temp. 35 °C
mobile phase [A] 0.005 M ammonium formate, pH 3.15 with concentrated TFA [B] acetonitrile (30:70, A:B)
flow rate 0.5 mL/min
pressure 841 psi (58 bar)
sample 0.5 mg/mL each prepared in 50:50 Water:Acetonitrile
injection 10 µL
detector ELSD (detector other: Nebulizer 60º C, Evaporator 80º C, Gas flow 1.60 SLM (Standard Liters/ Minute), LED 100%, Smoothing 5.0 S, PMT ( PhotoµLtiplier) Gain 1.0)

Description

Analysis Note This application shows the analysis of sugars by HILIC Chromatography with ELSD detection. The purpose of the work shown here was to develop a HILIC method that could quickly separate mono- and di-saccharides with good sensitivity and reproducibility utilizing the Ascentis Express OH-5 Column and an Evaporative Light Scattering Detecctor (ELSD) detector.
Categories Analytical Chromatography, Carbohydrates
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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