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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 15:85)

Conditions

column Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 µm particles (53981-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (15:85, A:B)
flow rate 1.0 ml/min
injection 10 µl
detector ELSD, 55 C, 3.5 bar nitrogen

Description

Analysis Note This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
Categories Analytical Chromatography, Carbohydrates
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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