HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles (53823-U)
column temp. 40 C
mobile phase [A] 100 mM ammonium phosphate monobasic, pH 7.0 with ammonium hydroxide; [B] water; [C] acetonitrile
gradient 10% A constant; 85 to 25% B, 5 to 65% C in 6 min; 25% B, 65% C held for 5 min
flow rate 0.3 ml/min
sample 20 µg/mL each in water
injection 5 µl
detector UV, 254 nm

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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