HPLC Analysis of Testosterone and 1-Testosterone on Ascentis® Express C18

HPLC Analysis of Testosterone and 1-Testosterone on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 4.6 mm I.D., 2.7 µm particles (53827-U)
column temp. 35 C
mobile phase [A] water; [B] methanol; (20:80, A:B)
flow rate 1 ml/min
sample 50 µg/mL in 10:90 water:methanol
injection 5 µl
detector UV, 240 nm

Description

Categories Analytical Chromatography, Clinical, steroids, Steroids, hormones
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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