HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Propionitrile Mobile Phase, ELSD Detection)

HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 after Sample Prep using Discovery® DSC-Si (Propionitrile Mobile Phase, ELSD Detection)

Conditions

SPE tube/cartridge Discovery DSC-Si, 1 gm/6 mL (52656-U)
condition 6 mLhexane
sample addition 0.12 g oil in 0.5 mL hexane
elution 10 mL hexane-diethyl ether 87-13 (v/v)
eluate post-treatment vaporation to dryness, reconstituted into 2 mL acetone
column Discovery HS C18, 25 cm x 4.6 mm I.D., 5 µm particles (568523-U)
column temp. 30 °C
mobile phase propionitrile
flow rate 1.5 mL/min
pressure 1117 psi (77 bar)
injection 5 µL
detector ELSD

Description

Analysis Note Soybean oil was cleaned using Silica SPE and analyzed as 5% sample in acetone (w/v).
Categories Analytical Chromatography, Fatty Acids, FAMEs, Glycerols
Featured Industry Food and Beverages
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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