HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

Conditions

column Discovery HS C18, 25 cm x 4.6 mm I.D., 5 µm particles (568523-U)
column temp. 30 C
mobile phase [A] acetone; [B] acetonitrile; (60:40, A:B)
flow rate 1.5 ml/min
pressure 914 psi (63 bar)
sample 5% soybean oil in acetone (w/v)
injection 5 µl
detector ELSD

Description

Analysis Note Soybean oil was analyzed as 5% diluted sample in acetone (w/v)
Categories Analytical Chromatography, Fatty Acids, FAMEs
Featured Industry Food and Beverages
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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