Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Triacylglycerols (TAG) of Borago Officinalis Oil on Ascentis® Express C18

HPLC Analysis of Triacylglycerols (TAG) of Borago Officinalis Oil on Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles (53829-U)
mobile phase [A] acetonitrile; [B] isopropyl alcohol
gradient 0% B held for 50 min; to 70% B in 5 min; to 0% B in 1 min
flow rate 1 ml/min
sample Borago Officinalis, 40 mg diluted in 10 mL of hexane
injection 2 µl
detector MS, APCI(+); mass spectral range, 250-1200 m/z; interval, 0.5 s; scan speed, 2143 amu/s; nebulizing gas (N2) flow, 4.0 L/min; interface temperature, 400 C; (heat block, 230 C; desolvation line (DL) temperature, 250 C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV)

Description

Categories Analytical Chromatography, Flavors, Fragrances
Featured Industry Flavors and Fragrances
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links