HPLC Analysis of Triazine Herbicides in Grass on SUPELCOSIL™ LC-8-DB after SPE using Supelclean™ LC-SCX

HPLC Analysis of Triazine Herbicides in Grass on SUPELCOSIL™ LC-8-DB after SPE using Supelclean™ LC-SCX

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix fresh grass clippings spiked with herbicides at 2 ppm
SPE tube/cartridge Supelclean LC-SCX, 500 mg/3 mL (57018)
condition 1 mL methylene chloride
sample addition 2 mL grass extract. Wash with 2 x 2 mL acetonitrile.
washing Dry packing for 5 min under nitrogen purge; wash with 2 x 2 mL DI water
drying Dry packing for 5 min. under nitrogen purge
elution 1.5 mL methanol
eluate post-treatment dilute to 2 mL with DI water
column SUPELCOSIL LC-8-DB, 15 cm x 4.6 mm I.D., 5 µm (58347-U) with guard column (59553)
mobile phase [A] acetonitrile; [B] water; (45:55, A:B)
flow rate 1.5 mL/min
injection 100 µL
detector UV, 254 nm

Description

Application Sample Pre-treatment:
5 g fresh grass clippings spiked with 2 ppm each herbicide. Add 4 g anhydrous sodium sulfate and 20 mL methylene chloride:acetone (80:20). Shake 20 min and allow mixture to stand 1 min.
Categories Analytical Chromatography, Environmental Analysis, SPE Applications, Herbicides, Organics, Chromatograms, HPLC Chromatograms
Featured Industry Environmental
Legal Information Supelclean is a trademark of Sigma-Aldrich Co. LLC, SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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