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HPLC Analysis of Tricyclic Antidepressants on Ascentis® ES Cyano

HPLC Analysis of Tricyclic Antidepressants on Ascentis® ES Cyano

Conditions

column Ascentis ES Cyano, 15 cm x 4.6 mm I.D., 5 µm particles (577306-U)
column temp. 25 C
mobile phase [A] 10 mM potassium phosphate dibasic, pH 7 with phosphoric acid; [B] acetonitrile; [C] methanol; (25:60:15, A:B:C)
flow rate 2 ml/min
sample 100 µg/mL in water: methanol (70:30)
injection 5 µl
detector UV, 215 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis ES Cyano for the efficient separation of tricyclic antidepressants.
Categories Analytical Chromatography, Antidepressants
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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