HPLC Analysis of tRNA Hydrolysate on SUPELCOSIL™ LC-18-S

HPLC Analysis of tRNA Hydrolysate on SUPELCOSIL™ LC-18-S

Conditions

column SUPELCOSIL LC-18-S, 15 cm x 4.6 mm I.D., 5 µm particles (58931)
column temp. 26 C
mobile phase [A] 0.1M potassium phosphate, dibasic (pH 5.3):methanol, 97.5:2.5; [B] 0.01M potassium phosphate, dibasic (pH 5.1):methanol, 80:20; [C] 0.01M potassium phosphate, dibasic (pH 4.9):acetonitrile, 65:35
flow rate 1 ml/min
injection 50 µl hydrolysate buffer (pH 7.8) containing 5 mug bovine tRNALeu hydrolysate
detector UV, 210 nm, 254 nm, 280 nm

Description

Categories Analytical Chromatography, Nucleic Acids
Featured Industry Life Science and Biopharma
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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