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HPLC Analysis of Tryptic Digest on BIOshell™ A160 Peptide C18

HPLC Analysis of Tryptic Digest on BIOshell™ A160 Peptide C18

Conditions

column BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (66905-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water; [B] 25:75, (0.4% formic acid in water):acetonitrile
gradient 0 to 70% B in 40 min.
flow rate 0.3 ml/min
sample 10 pg/L in 0.1% formic acid
injection 2 µl
detector ESI(+), TOF

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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