HPLC Analysis of Ulcer Treatment Drugs on Ascentis® RP-Amide

HPLC Analysis of Ulcer Treatment Drugs on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 µm particles (565305-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium phosphate, pH 6.2 with phosphoric acid; [B] acetonitrile
gradient 5 to 15% B in 10 min; to 55% B in 8 min; held for 5 min
flow rate 0.2 ml/min
sample as indicated in 95:5 water:methanol
injection 5 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Antiulcer Compounds
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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