HPLC Analysis of Vinblastine Sulfate on Chromolith® HighResolution RP-18 endcapped 150 x 4.6mm

HPLC Analysis of Vinblastine Sulfate on Chromolith® HighResolution RP-18 endcapped 150 x 4.6mm

Conditions

sample preparation Transfer USP Vincristine Sulfate RS  ( or Assay RS) to a suitable volumetric flask, and dissolve in the Standard solution.
column Chromolith(R) HighResolution RP-18 endcapped, 150 x 4.6 mm (Merck p/n 1.52023) (1.52023.0001)
column temp. ambient
mobile phase [A] Dimethylamine and water 14/986 (v/v). Adjusted with phosphoric acid to a pH of 7.5; [B] Acetonitrile and Methanol 20/80 (v/v); (38:62, A:B)
flow rate 2.0 ml/min
pressure 2030 psi (140 bar)
sample 0.4 mg/mL each of Vincristine sulfate  and Vinblastine Sulfate in water (Diluent: water)
injection 20 µl
detector cell: 11 µl
detector 262 nm

Description

Featured Industry Pharmaceutical (small molecule)
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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