HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

Conditions

column Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 µm particles (53825-U)
column temp. 35 °C
mobile phase acetonitrile
flow rate 0.15 mL/min
sample 10 mg/L each (vitamins D2 and D3) in methanol
injection 10 µL
detector UV, 290 nm

Description

Categories Analytical Chromatography, Clinical, vitamins, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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