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HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18


column Ascentis(R) Express C18, 25 cm x 4.6 mm I.D., 5 µm particles (50538-U)
column temp. 35 C
mobile phase [A] acetonitrile; [B] methanol; (95:5, A:B)
flow rate 1.5 ml/min
pressure 1726 psi (119 bar)
sample 50 µg/mL in 90:10, acetonitrile:methanol
injection 10 µl
detector UV, 265 nm


Analysis Note This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.
Categories Analytical Chromatography, Vitamins and Natural products, Steroids
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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