HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

Conditions

column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 C
mobile phase acetonitrile
flow rate 1.0 ml/min
sample 50 µg/mL each in acetonitrile
detector UV, 290 nm

Description

Analysis Note This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.
Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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