HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

Conditions

column SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
mobile phase  [A] methanol: [B] deionized water (98:2, A:B)
flow rate 2 mL/min
detector UV, 325 nm (retinol, retinyl acetate) or 290 nm (tocopherols, tocopherol acetate)
injection: 20 μL
sample 0.2-1 mg/mL each analyte in methanol

Description

Featured Industry Clinical Vitamins, Nutraceuticals, and Natural Products
Legal Information SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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